FIG. 4.

Self-association of Pind protein is not required for its binding to N and L proteins. HA-Pind, HA-Δ161-210, or HA-ΔII+III was coexpressed with Myc-Nind (A) or Flag-L (B) in HeLa cells as indicated. Cell lysates were assayed for protein expression by Western blotting (WB) using anti-Myc and anti-HA monoclonal antibodies (A) or anti-Flag and anti-HA monoclonal antibodies (B). Immunoprecipitation (IP) was performed using anti-Myc (A) or anti-L (B) polyclonal antibody, and proteins in immune complexes were detected using anti-Myc and anti-HA (A) or anti-Flag and anti-HA (B) monoclonal antibodies.