TABLE 1.
Expt no. | Marker distance (bp) | No. of cells
|
MOI
|
Recombination ratea (%) | % of TMMRRb | |||
---|---|---|---|---|---|---|---|---|
Total | Infected | GFP+ | Infection | GFP | ||||
1 | 90 | 361,134 | 16,382 | 405 | 0.052 | 0.00112 | 2.2 | 17.2 |
288 | 421,556 | 18,611 | 1,030 | 0.045 | 0.00244 | 5.4 | 43.4 | |
588 | 238,995 | 14,164 | 1,010 | 0.061 | 0.00424 | 7.0 | 55.6 | |
2 | 90 | 320,226 | 13,996 | 332 | 0.045 | 0.00103 | 2.3 | 18.3 |
288 | 326,315 | 14,756 | 725 | 0.046 | 0.00222 | 4.8 | 38.6 | |
588 | 409,408 | 19,490 | 1,361 | 0.049 | 0.00333 | 6.8 | 54.4 | |
3 | 90 | 669,949 | 7,659 | 197 | 0.011 | 0.00029 | 2.6 | 21.1 |
288 | 531,655 | 7,533 | 463 | 0.014 | 0.00087 | 6.2 | 49.7 | |
588 | 570,511 | 13,037 | 1,086 | 0.023 | 0.00191 | 8.3 | 66.4 |
Means ± standard deviations for markers separated by the indicated distances were as follows: 90 bp, 2.4% ± 0.2%; 288 bp, 5.5% ± 0.6%; 588 bp, 7.4% ± 0.7%.
Means ± standard deviations for markers separated by the indicated distances were as follows: 90 bp, 18.9% ± 1.6%; 288 bp, 43.9% ± 4.5%; 588 bp, 58.8% ± 5.4%.