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TABLE 1.

HIV-2 recombination rates in Hut/CCR5 cells

Expt no. Marker distance (bp) No. of cells
MOI
Recombination ratea (%) % of TMMRRb
Total Infected GFP+ Infection GFP
1 90 361,134 16,382 405 0.052 0.00112 2.2 17.2
288 421,556 18,611 1,030 0.045 0.00244 5.4 43.4
588 238,995 14,164 1,010 0.061 0.00424 7.0 55.6
2 90 320,226 13,996 332 0.045 0.00103 2.3 18.3
288 326,315 14,756 725 0.046 0.00222 4.8 38.6
588 409,408 19,490 1,361 0.049 0.00333 6.8 54.4
3 90 669,949 7,659 197 0.011 0.00029 2.6 21.1
288 531,655 7,533 463 0.014 0.00087 6.2 49.7
588 570,511 13,037 1,086 0.023 0.00191 8.3 66.4
a

Means ± standard deviations for markers separated by the indicated distances were as follows: 90 bp, 2.4% ± 0.2%; 288 bp, 5.5% ± 0.6%; 588 bp, 7.4% ± 0.7%.

b

Means ± standard deviations for markers separated by the indicated distances were as follows: 90 bp, 18.9% ± 1.6%; 288 bp, 43.9% ± 4.5%; 588 bp, 58.8% ± 5.4%.