FIG. 1.

zVAM.fmk inhibits HRV replication. (A) HeLa cells were infected with 100 TCID₅₀ units per cell in the absence (black bars) or in the presence (gray bars) of 200 μM zVAM.fmk, and the virus titers were determined 12 h p.i. Means and standard deviations were calculated from three separate experiments. (B) HeLa cells were infected with 100 TCID₅₀ units per cell in the presence of the indicated concentrations of zVAM.fmk, and the virus titers were determined 12 h p.i. (C) HeLa cells were infected as described above at 90% confluence in the absence or presence of 200 μM zVAM.fmk, and phase-contrast photographs were taken 12 h p.i at ×2,000 magnification. (D) HeLa cells were preincubated for 30 min with 200 μM zVAM.fmk before infection with HRV2 or HRV14 (100 TCID₅₀ units per cell) as indicated. Cell viability was determined 24 h p.i. with the proliferation assay described in Materials and Methods. Cellular metabolic activity is shown as a percentage of the control (mock-infected cells = 100%). The results were calculated from three separate experiments.