TABLE 1.

Integrity of nuclei determined by their exclusion of dextran-TRITC

Description of cells or nuclei	Value for incubation		% Impermeable material
	Time (h)	Temp (oC)
Control cells	0		95 ± 0
Permeabilized cellsa	0		0 ± 0
Wild-type nuclei	0		86 ± 6
	6	4	88 ± 10
	6	37	82 ± 10
V701 nuclei	0		62 ± 2
	6	4	63 ± 6
	6	31	72 ± 9
	6	39.5	66 ± 5

^aCells were treated for 10 min with 0.1% Triton X-100 on ice.