FIG. 6.

Delayed expression of VZV gC in cultured Vero cells. Vero cell monolayers were inoculated with cells prepared from a trypsin-dispersed VZV-infected Vero cell monolayer with advanced CPE. Cultures were examined at 24, 48, 72, and 96 hpi, after immunolabeling with mouse anti-gE and rabbit 233 anti-gC antibodies. The representative syncytium at 72 hpi with abundant gE staining demonstrated that VZV had undergone several cycles of replication. In contrast, only a small number of cells stained positively for gC; the above gC-enriched focus represented the original inoculum.