FIG. 3.

HLA-DR redirects Gag to LE/MVB in HEK 293T cells. HEK 293T cells were transfected with the proviral construct HxBc2, together with HLA-DR or empty vector. Gag and MVB markers were detected by immunofluorescence microscopy 48 h later using a rabbit polyclonal anti-p24 antibody together with monoclonal antibodies against MVB markers. In the absence of HLA-DR, Gag shows primarily a diffuse staining (left panels of A, B, and C). Upon HLA-DR expression, Gag accumulates into Lamp1-positive (A), CD63-positive (B), and LBPA-positive (C) compartments (right panels).