Skip to main content
. 2006 Oct;80(19):9697–9709. doi: 10.1128/JVI.00746-06

FIG. 1.

FIG. 1.

Transcriptionally deficient Rta restores the ability of constitutively active RBP-Jk to transactivate KSHV genes in a promoter-specific manner. (A) Sequence alignment of the RBP-Jk-containing elements of the indicated promoters and the cloning strategy for each element in the reporter vector. Numbers represent the positions of each sequence relative to the transcriptional start site (Mta and Cp) or ORF (TK only) of each gene. (B and C) Expression vectors pCMX-VP16-RBP-Jk (expressing RBP-Jk/VP16) and pcDNA3.1-Myc-HisC-NICD (expressing NICD) were cotransfected into BL-41 cells with reporter plasmids p57Δ5-5Dwt-hsp-luc (B) and 2xRBP-hsp-luc (C) as indicated. Calculations are described in Materials and Methods. Error bars represent standard deviations of the means of at least three experiments. (D to G) Expression vectors V5-50ΔSTAD and those from panels B and C were cotransfected into BL41 cells (D, F, and G) or BJAB cells (E) with reporter plasmids p57Δ5-5Dwt-hsp-luc (D and E), p57WT-GL3 (F), and pTK-WT-hsp-luc (G). Extracts were analyzed as described above. For BL41 cells, + or ++ represents 3 or 9 μg of V5-50ΔSTAD, respectively. For BJAB cells, +, ++, or +++ represents 1, 3, or 9 μg of V5-50ΔSTAD, respectively. −, none.