TABLE 2.

Specific titers of broadly flavivirus cross-reactive MAbs in ELISA and HI and NT assays

MAb	ELISA titera				HI titerb				NT90 titerc		NT50 titerc
	TBEV	JEV	WNV	DENVd	TBEV	JEV	WNV	DENV-2	TBEV	JEV	TBEV	JEV
A1	208,900	513,300	733,300	154,800	512	2,048	4096	2,048	<1	<1	2-4e	4-8e
A2	235,200	75,700	41,900	40	512	512	32	<1	<1	<1	<1	<1
IA3	1,200	2,700	3,700	300	512	2,048	4096	2048	<1	1-2	1-2e	4-8
IF3	83,800	306,700	305,600	200,000	512	2,048	2,048	8192	<1	<1	2-4e	4-8e
IN3	35,400	124,100	150,000	100,000	512	1,024	1,024	4096	<1	<1	<1	4-8e
IO3	4,600	4,900	4,600	5,000	8	2	64	256	<1	<1	<1	<1
IS3	6,900	12,300	11,400	5,000	1,024	2,048	2,048	2,048	<1	<1	<1	<1
4G2	26,600	118,600	144,400	83,300	256	512	512	512	1-2	1-2	4-8	4-8
843	200	1,600	800	200	2	16	2	2	<1	<1	<1	<1
813	200	5,200	5,000	80	2	4	4	2	<1	<1	<1	<1
528	800	2,400	2,700	2,000	16	64	64	16	<1	<1	<1	<1
612	11,200	119,600	105,500	100,000	256	2048	1,024	512	<1	<1	<1	<1
F7/3	2,600	28,800	26,700	2,000	256	1,024	512	1,024	<1	1-2	1-2e	4-8
6B6C1	32,600	410,900	444,400	333,330	512	2,048	1,024	2,048	<1	<1	<1	<1

^aSpecific titer for a MAb protein concentration of 100 μg/ml (cutoff for determination of titration endpoint, optical density of 0.1 at 490 nm); data are expressed as the average titer obtained in at least two (DENV) or four (TBEV, JEV, and WNV) independent experiments.

^bSpecific titer for a MAb protein concentration of 100 μg/ml; data from at least two independent experiments.

^cSpecific titer for a MAb protein concentration of 500 μg/ml; data from at least two independent experiments.

^dThe ELISA antigen was a mixture of dengue virus types 1, 2, 3, and 4 (see Materials and Methods).

^eEven at the highest MAb protein concentration (500 μg/ml), only partial inhibition of virus replication was observed.