FIG. 7.

Comparison of the composition of wild-type 22/n199 and 22/n199R virions. Vero cell monolayers were infected with wild-type (wt), 22/n199 (9), and 22/n199R (R) viruses, or V22 cells were infected with 22/n199 (V). Cellular lysates, extracellular virions, and purified extracellular virions were prepared, solubilized in RIPA buffer, and resolved on 10% SDS-polyacrylamide gels. (A) Protein profile visualized by silver staining. Proteins unique to purified 22/n199 preparations are marked by asterisks. (B and C) Western blot analysis against the proteins indicated on the left. In the upper ICP0 Western blot a quantity of ICP0 was present that was sufficient to quench the signal in 22/n199 virion preparations. The bottommost ICP0 blot contains 1/10 the amount of protein loaded in each lane. MW, molecular weights in thousands.