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. 2006 Oct;80(20):10036–10044. doi: 10.1128/JVI.00186-06

FIG. 1.

FIG. 1.

Overexpression of CARM1 increases Tax transcriptional activity of the HTLV-1 LTR. (A to C) HeLa cells were transiently transfected using Effectene transfection reagent (QIAGEN) with reporter construct (0.1 μg) HTLV-1 LTR-Luc (A) or GEM- or GEM(del CREM)-Luc (C), RSV β-Gal (0.05 μg), CARM1 (0.1, 0.2, or 0.4 μg), or Tax (0.1 μg) expression plasmids. At 24 h posttransfection, cells were collected, and luciferase activities were measured. (B) Western blot analysis was performed for Tax (Tab172) and CARM1 (Upstate). (D and E) Jurkat and Molt-4 cells were transfected using Superfect transfection reagent (QIAGEN) with HTLV-1 LTR-Luc (1 μg), RSV β-Gal (0.5 μg), CARM1 (1 or 2 μg), or Tax (1 μg) expression plasmids. All luciferase values were adjusted for transfection efficiency using RSV β-Gal. The graph represents the luciferase activity from three independent experiments. The standard deviation for the three experiments is included.