FIG. 2.

CARM1 mutant does not influence Tax transcription of the HTLV-1 LTR. (A) HeLa cells were transiently transfected using Effectene transfection reagent (QIAGEN) with reporter construct HTLV-1 LTR-Luc (0.1 μg), RSV β-Gal (0.05 μg), CARM1 WT or MT (0.1 μg), or Tax (0.1 μg) expression plasmids. At 24 h posttransfection, cells were collected and luciferase activities were measured. Luciferase values were adjusted for transfection efficiency using RSV β-Gal. The graph represents the luciferase activity from three independent experiments. The standard deviation for the three experiments is included. (B) Western blot analysis was performed for Tax (Tab172) and CARM1 (Upstate).