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CARM1 is associated with active HTLV-1 LTR chromatin in vivo. SP cells, which contain a single active integrated copy of the HTLV-1 proviral genome, were subjected to ChIP assays. Antibodies specific for Tax, CARM1, dimethyl histone H3 (R2, R17, R26, or K9), histone H3, and acetylated histone H3 (K9) were used for immunoprecipitation. PCRs were carried out to analyze precipitated DNA using primers specific for the HTLV-1 LTR and β-globin promoter region.
