FIG. 5.
Viremia, anti-FrCasE immunoglobulins, and FrCasE RNA in challenged group I to III mice. (A) Viremia. FrCasE was administered to three mice from each group and three control animals in week 30. Serum samples were collected at different times for 18 days, and viremia was assayed by FIA. (B) Assay of anti-FrCasE IgGs. IgGts were assayed by ELISA. The values are presented as the mean of the values obtained for each mouse of each group ± standard error of the mean. (C) FrCasE Env mRNA in the spleen. The presence of Env mRNA was assayed by RT-PCR in total spleen RNAs from animals sacrificed 18 days postinfection. β-Actin was used as an internal standard of amplification.