FIG. 3.

Effects of vaccinia virus infection on constitutive and LPS-induced cytokine production by XS52 cells. XS52 cells (6 × 10⁶) were infected with WT WR or ΔE3L vaccinia virus at a multiplicity of 10 or mock infected. At 2 h after removal of the inoculum, LPS was added to the medium (where indicated) to a concentration of 1 μg/ml. The concentrations of secreted TNF-α (A) and IL-6 (B) in the medium at the indicated times thereafter were determined by ELISA. Each datum represents the average of three experiments with standard deviations shown.