FIG. 7.

Productive replication of the ΔK1L virus in XS52 cells and effects of K1L deletion on LPS-induced cytokine production. (A) XS52 cells (6 × 10⁶) were infected with WT WR or ΔK1L vaccinia virus at a multiplicity of 3. The inoculum was removed after 30 min. Cells were harvested at the indicated times postinoculation. Virus yield (log PFU) was determined by titration on BSC40 cell monolayers. (B and C) XS52 cells (6 × 10⁶) were infected with WT WR or ΔK1L vaccinia virus at a multiplicity of 10 or mock infected. At 2 h after removal of the inoculum, LPS was added to the medium (where indicated) to a concentration of 1 μg/ml. The concentrations of secreted TNF-α (A) and IL-6 (B) in the medium at the indicated times thereafter were determined by ELISA. Each datum represents the average of three experiments with standard deviations shown.