FIG. 4.

Determination of optimal conditions for membrane association of in vitro-translated Vhs. (A to C) In duplicate experiments, in vitro-translated Vhs1 was incubated with infected-cell PNS for 1 h at 4°C (A), room temperature (B), and 37°C (C) before being subjected to sucrose density gradient centrifugation. The membrane fractions (lanes 1 and 3) and the rest of the gradient (lanes 2 and 4) were analyzed by SDS-PAGE. (D) In vitro-translated Vhs1 was incubated with infected-cell PNS at 37°C for various times before separation of membranes by flotation. The membrane fractions obtained were analyzed by SDS-PAGE.