FIG. 8.
D1(42)DsRed2 colocalizes with endogenous Vhs. COS cells grown on coverslips were either transfected with D1(42)DsRed2 (A and G to J) or DsRed2 (B) or were mock transfected (C to F) and subsequently infected with virus strain N138HA (C, D, and G to J) or PAAR5 (E and F). The cells were fixed in 4% paraformaldehyde and immunostained for HA as described in Materials and Methods. The images were merged and pseudocolored using Image J software. Panels G and H are the separate green and red channel images of the same field, which has been merged in panel I. Panels D, F, and J are the phase-contrast images of panels C, E, and I, respectively.