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Viral-gene expression and DNA replication. NIH 3T3 cells were infected with recombinant MCMVs at an MOI of 0.5 TCID50/cell. Viral-gene products were detected with specific antibodies 24 (A) and 72 (B) h p.i. Detection of viral-DNA amplification in infected cells by slot blotting and hybridization with an MCMV-specific probe 24 (C) and 72 (D) h p.i. βa, beta actin; wt, wild type; mock, mock infected.
