FIG. 6.

Shutdown of protein synthesis in Δm142- and Δm143-infected cells. (A) NIH 3T3 cells were metabolically labeled with [³⁵S]methionine and [³⁵S]cysteine 24 h p.i. at an MOI of 5 TCID₅₀/cell. Cell lysates (10 μg total protein per sample) were separated by gel electrophoresis and analyzed by autoradiography. (B) Detection of phosphorylated PKR and eIF2α (P-eIF2α) in Δm142- and Δm143-infected cells by Western blotting. Total eIF2α and β-actin were used as controls. mock, mock infected; wt, wild type.