FIG. 8.

TRS1 rescues m142 and m143 deletion mutants. (A) Multistep growth kinetics of the wild type (wt) and deletion mutants on NIH 3T3 cells stably transduced with a TRS1-expressing retroviral vector. (B) Introduction of the HA-tagged TRS1 gene into the MCMV genome, either by replacing the deleted gene or (C) by insertion at a distant position together with a pgk promoter. (D) Multistep growth curves of the TΔm142 and TΔm143 mutants on NIH 3T3 fibroblasts. (E) Viral-protein expression 24 and (F) 72 h p.i. at an MOI of 0.5 TCID₅₀/cell. The error bars represent the standard errors.