TABLE 3.

Detection of betanodavirus RNA2 in infected fish^a

Trial	Vaccine	Treatment or VLP dose (μg · fish−1)	No. of fish with VNN clinical signs positive by RT-PCR/no. of fish examined	No. of dead fish positive by RT-PCR/no. of fish examined	No. of asymptomatic fish positive by RT-PCR/no. of fish examined
1	MGNNV VLPs	20		NDb	12/44
		100	1/1	2/54
		Unvaccinated	3/3	1/4	1/5
2	SB2 VLPs	0.1	3/3	14/14	14/15
		0.5	1/1	18/18	15/15
		1	2/2	9/9	14/15
		5	4/4	14/15
		10	4/4	11/15
		20	1/1	3/3	6/15
		Unvaccinated	9/9	17/17	17/17

^aRT-PCR analysis was performed using RNA extracted from the eyes and brains of challenged fish as described in Materials and Methods.

^bND, not determined.