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. 2006 Oct;80(20):10218–10228. doi: 10.1128/JVI.00375-06

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Copyright © 2006, American Society for Microbiology

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FIG. 7. — (A) Overexpression of HHV-6A IE2 does not alter Ubc9 expression or the sumoylation pattern of total cellular proteins. HEK293T cells were transfected with 2, 4, or 6 μg pcDNA4-IE2 and 2 μg HA-tagged construct pCMV-SUMO-1. Cells were harvested 48 h after transfection. Total lysates were analyzed by SDS-PAGE and probed for Ubc9, IE2, HA-tagged sumoylated proteins (designated by a bracket), and actin. (B) Overexpression of HHV-6A IE2 does not alter the sumoylation pattern of RanGAP1. HEK293T cells were transfected with 2 or 4 μg pcDNA4-IE2, 2 μg pCMV-SUMO-1, and 2 μg pcDNA3-Myc-RanGAP1. Total lysates were analyzed by SDS-PAGE and probed for RanGAP1 using an anti-Myc antibody. Arrows indicate (from top to bottom) HA-SUMO-1-modified Myc-RanGAP1, endogenous SUMO-1-modified Myc-RanGAP1, and unconjugated Myc-RanGAP1. HA-tagged SUMO-1 is 26 kDa, while endogenous SUMO-1 is 18 kDa.