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. 2006 Oct;80(20):10218–10228. doi: 10.1128/JVI.00375-06

TABLE 1.

Mapping of the IE2-Ubc9 interaction domain by yeast two-hybrid screening

Bait (pHybLex fusion) Prey (pYESTrp fusion)
Ubc9
Ubc9 C93S
His-negative growtha β-Gal activityb His-negative growtha β-Gal activityb
Control (empty vector)c − 1.00 ± 0.38 − 1.02 ± 0.17
IE2 1-720 + 35.6 ± 7.62* + 38.8 ± 7.30*
IE2 925-1466 + 9.40 ± 2.89* + 14.6 ± 2.31*
IE2 925-1233 + 7.73 ± 2.41* + 10.0 ± 1.92*
IE2 925-1176 + 4.23 ± 1.48* + 6.00 ± 1.19*
IE2 925-1131 + 4.09 ± 1.74* + 6.81 ± 1.34*
IE2 925-1083 + 7.46 ± 2.25* + 7.89 ± 1.64*
IE2 925-1037 + 14.4 ± 2.83* + 20.9 ± 4.50*
IE2 925-988 − 1.45 ± 0.51 − 1.52 ± 0.40
a

A + indicates growth, while a − indicates that there was no growth.

b

Results are expressed as means ± standard deviations from three distinct experiments. Reference activity was that of control cells cotransformed with pYESTrp-Ubc9 and pHybLex. Asterisks indicate that P was <0.05 compared with reference activity. β-Gal, β-galactosidase.

c

Control cotransformations with empty prey vector pYESTrp and each of the bait constructs were all negative for His-negative growth except for pHybLex-IE2 1-720 (β-Gal activity = 41.0 ± 10.8; P < 0.05). Cotransformations with prey pYESTrp-SUMO-1 and each of the bait constructs were all negative for His-negative growth, except for pHybLex-IE2 1-720 (β-Gal activity = 39.5 ± 11.1; P < 0.05) and pHybLex-Ubc9 (12.1 ± 2.05; P < 0.05).