TABLE 1.
Bait (pHybLex fusion) | Prey (pYESTrp fusion)
|
|||
---|---|---|---|---|
Ubc9
|
Ubc9 C93S
|
|||
His-negative growtha | β-Gal activityb | His-negative growtha | β-Gal activityb | |
Control (empty vector)c | − | 1.00 ± 0.38 | − | 1.02 ± 0.17 |
IE2 1-720 | + | 35.6 ± 7.62* | + | 38.8 ± 7.30* |
IE2 925-1466 | + | 9.40 ± 2.89* | + | 14.6 ± 2.31* |
IE2 925-1233 | + | 7.73 ± 2.41* | + | 10.0 ± 1.92* |
IE2 925-1176 | + | 4.23 ± 1.48* | + | 6.00 ± 1.19* |
IE2 925-1131 | + | 4.09 ± 1.74* | + | 6.81 ± 1.34* |
IE2 925-1083 | + | 7.46 ± 2.25* | + | 7.89 ± 1.64* |
IE2 925-1037 | + | 14.4 ± 2.83* | + | 20.9 ± 4.50* |
IE2 925-988 | − | 1.45 ± 0.51 | − | 1.52 ± 0.40 |
A + indicates growth, while a − indicates that there was no growth.
Results are expressed as means ± standard deviations from three distinct experiments. Reference activity was that of control cells cotransformed with pYESTrp-Ubc9 and pHybLex. Asterisks indicate that P was <0.05 compared with reference activity. β-Gal, β-galactosidase.
Control cotransformations with empty prey vector pYESTrp and each of the bait constructs were all negative for His-negative growth except for pHybLex-IE2 1-720 (β-Gal activity = 41.0 ± 10.8; P < 0.05). Cotransformations with prey pYESTrp-SUMO-1 and each of the bait constructs were all negative for His-negative growth, except for pHybLex-IE2 1-720 (β-Gal activity = 39.5 ± 11.1; P < 0.05) and pHybLex-Ubc9 (12.1 ± 2.05; P < 0.05).