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. 2006 Oct 5;25(20):5015–5025. doi: 10.1038/sj.emboj.7601367

Figure 1.

Figure 1

Expression of FKBP8 and FKBP38 in mammalian cells. (A) Schematic representation of FKBP8 and FKBP38. The FK506-binding domain (FBD), tetratricopeptide repeat (TPR), putative calmodulin binding motif (CaM), and transmembrane domain (TM) are shown. (B) N-terminally HA-tagged FKBP8 and FKBP38 were expressed in 293T cells and visualized by immunoblotting using mouse monoclonal antibody to FKBP8 or the HA tag. (C) HA-FKBP8 was expressed together with Flag-NS5A of genotype 1b (J1) in 293T cells and was immunoprecipitated with anti-HA antibody. Immunoprecipitated proteins were subjected to immunoblot with anti-Flag or HA antibody. (D) Endogenous FKBP8 in HCV replicon (9–13) cells was immunoprecipitated with isotype control (lane 1) or anti-FKBP8 antibody, KDM-11 (lane 2). Endogenous FKBP8 was co-immunoprecipitated with HCV NS5A. The data shown in each panel are representative of three independent experiments.