Figure 6.

Expression of nestin deletion mutants protects ST15A cells from oxidant-induced cell death. Apoptosis of ST15A cells expressing the GFP-tagged nest-1177, nest-640, as compared to control cells, was determined by chromatin condensation visualized by DAPI-labeling and analyzed with immunofluorescence microscopy. Arrows denote cells with apoptotic nuclear morphology not expressing the nestin constructs and asterisks denote apoptotic cells expressing the nestin peptides. The graph represents the mean value of four different fields in three different experiments.