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. 2006 Oct 12;25(20):4808–4819. doi: 10.1038/sj.emboj.7601366

Figure 9.

Figure 9

Overexpression of a soluble nestin mutant affects the turnover and ubiquitylation of p35. (A) HA-tagged ubiquitin, p35, and nest-640 were transfected into ST15A cells as indicated and cells were treated with and without MG-132. Cell lysates (Lysate) were run directly on SDS–PAGE or immunoprecipitated with anti-HA antibody (IP) and immunoblotted with a p35 antibody. (B) ST15A cells were transfected with p35 with or without nest-640 and the half-life of p35 was analyzed by a CHX chase assay. Cell lysates were separated on SDS–PAGE and blotted with anti-p35 (upper panel). The graph shows quantification of the relative half-life of p35 from samples transfected with p35 alone or together with nest-640 (lower panel).