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. 2006 Sep 21;25(20):4983–4995. doi: 10.1038/sj.emboj.7601349

Figure 2.

Figure 2

The PRD of SPIN90/WISH interacts with the SH3 domain of PSD-95 in vitro. (A, B) Schematic diagrams of the series of truncated GST-SPIN90/WISH and GST-PSD-95 constructs used in GST pull-down assays. (C) GST alone, GST-tagged full-length SPIN90/WISH or truncated variants were expressed in BL-21, then immobilized on glutathione-Sepharose beads and incubated with rat brain lysates. Bound proteins were analyzed by SDS–PAGE and immunoblotted with PSD-95 antibody. (D) After expression in BL-21, the GST-PSD-95 and truncated variants were immunoblotted with SPIN90/WISH antibody.