Skip to main content
. 2006 Sep 21;25(20):4983–4995. doi: 10.1038/sj.emboj.7601349

Figure 3.

Figure 3

Overexpression of SPIN90/WISH increases the number and length of dendritic filopodia in early hippocampal neurons. Neurons were transfected with various constructs, fixed at 7DIV and immunostained with GFP antibody. (A) GFP fluorescence images of the transfected neurons. High-magnification views are of the regions enclosed in rectangles. Images are inverted for clarity. Scale bars, low magnification: 20 μm; high magnification: 5 μm (B, C) Histograms of the number of dendritic filopodia per μm in the transfected neurons (B); comparison of average numbers of filopodia (C). Data are means±s.e. (GFP, n=9; SPIN90/WISH, n=8; SPIN90/WISH-ΔPRD, n=11; SPIN90/WISH-PRD, n=9; SPIN90/WISH-ΔSH3, n=10; SPIN90/WISH-SH3, n=8; ***P<0.01, significantly different from GFP alone by ANOVA and Tukey's HSD post hoc test). (D) Cumulative frequency distribution of dendritic filopodial length in the transfected neurons.