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. 2006 Sep 21;25(20):4983–4995. doi: 10.1038/sj.emboj.7601349

Figure 6.

Figure 6

SPIN90/WISH expression increases the number of synapses as well as the density of dendritic spines. (A–D) Neurons were transfected with various GFP-tagged SPIN90/WISH variants and immunostained using GFP (green) and synaptobrevin 2 (red) antibodies. Middle panels are merged images. High-magnification views of the regions enclosed by rectangles are shown below each image. Scale bars, low magnification:, 30 μm, high magnification: 5 μm. (E) Quantification of the number of synapses (gray bars) that contact GFP-positive dendritic spines (black bars). Data are means±s.e. (GFP, n=6; SPIN90/WISH, n=6; SPIN90/WISH-ΔPRD, n=7; SPIN90/WISH-PRD, n=6; ***P<0.01 by ANOVA and Tukey's HSD post hoc test). (F) Correlation between spinogenesis and synaptogenesis. The ratio of synapse density to spine density is displayed.