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. 2006 Sep 21;25(20):4983–4995. doi: 10.1038/sj.emboj.7601349

Figure 8.

Figure 8

The effect of PSD-95 on dendritic spinogenesis is mediated by SPIN90/WISH. (A, B) Neurons were transfected with GFP alone or PSD-95-GFP and visualized at 17DIV by immunoassaying for GFP. High-magnification views are of the regions enclosed in rectangles. The average number of spines per μm was analyzed (GFP, n=5; PSD-95-GFP, n=8; **P<0.05, Student's t-test). Scale bars, low magnification: 20 μm, high magnification: 5 μm (C, D) Neurons were cotransfected with PSD-95-HA and psiRNA-virgin, or with PSD-95-HA and psiRNA-SPIN90/WISH, and immunostained at 17DIV with HA and GFP antibodies. Only GFP images are shown. High-magnification views are of the regions enclosed in rectangles. Average number of spines per μm are presented (psiRNA-virgin/PSD-95-HA, n=6; psiRNA-SPIN90/WISH/PSD-95-HA, n=7; ***P<0.01, Student's t-test).