Table 1.

Purification of RK(His)₆ produced in transiently transfected COS-1 cells

RK(His)6	Vol, ml	Total Protein, mg	Specific Activity, nmol/min/mg	Purification, fold	Yield, %
In the absence of ATP
Crude	20	120	0.18	1	100
Heparin-sepharose	14	14	1.27	7	82
Cobalt-sepharose	10	0.4	40	222	74
In the presence of ATP
Crude	20	102	0.19	1
Heparin-sepharose	20	8	4.9	26	48
Cobalt-sepharose	8	0.2	40	210	43