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. 2006 Sep 15;7(10):1052–1058. doi: 10.1038/sj.embor.7400806

Figure 1.

Figure 1

serrate-1 decreases the accumulation of mature microRNAs and increases the accumulation of primary microRNA transcripts. (A) Northern blotting of total RNA from wild-type and serrate-1 (se-1) seedlings. For all microRNAs (miRNAs) tested (miRJAW, miR171, miR168, miR156, miR163, miR167, miR165, miR164), the se-1 mutant showed a reduced level of miRNA accumulation compared with wild type. (B) Accumulation of short interfering RNA (siRNA) in se-1 compared with wild type. The se-1 mutation had little effect on the accumulation of these siRNAs. The corresponding U6 loading control is shown below each blot. (C) Reverse transcription–PCR assay comparing levels of primary miRNAs (pri-miRNAs) in wild type and se-1. PCR products were blotted and hybridized with random-primed probes. The UBIQUITIN (UBQ) loading control is shown below the blots.