Table 1.
Spectral characteristics and parameters of refolding and refolding/maturation kinetics
| Fluorescent protein | ɛ (λmax)* | QY (λmax)‡ | Refolding half-time (s) | Maturation half-time (s) | kox (10−4 s−1) |
|---|---|---|---|---|---|
| EGFP |
55,000 (489) |
0.60 (509) |
90.6 |
3,915 |
1.77 |
| Venus§ |
110,000 (515) |
0.63 (527) |
46.2 |
4,076 |
1.70 |
| SYFP2§ |
101,000 (515) |
0.68 (527) |
69.3 |
3,300 |
2.10 |
| TurboGFP |
70,000 (482) |
0.53 (502) |
11.0 |
1,468 |
4.72 |
| TurboGFP-V197L | 73,000 (482) | 0.47 (502) | 9.5 | 2,493 | 2.78 |
Protein refolding and maturation were followed by measuring the recovery of fluorescence at 25°C. Maturation rate constants (kox) were determined by computer-fitting the kinetic data to the first-order exponential decay (Origin 6.0).
EGFP, enhanced green fluorescent protein; GFP, green fluorescent protein.
*Extinction coefficient (M−1 cm−1) with excitation maximum (nm) in parentheses.
‡Quantum yield with emission maximum (nm) in parentheses.
§Data from Kremers et al (2006).