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Kinetics of binding of opsin, N-terminal fragments of rhodopsin, and rhodopsin to the antibody B6–30N in different detergents. (A) Binding of opsin and N-terminal fragments: 35S-labeled M1-P142 (I), 3H-labeled N2-M39 (II), and opsin (III), respectively. (B) Binding of rhodopsin: binding of A500 (I) and binding of 35S-labeled rhodopsin (II). The detergents used were DM (open circles), cholate (closed circles), and a mixture of DM (0.1%) and cholate (2% vol/vol) (triangles).
