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TLC analysis of residual PLs in rhodopsin after purification and extensive washing with different detergents (details in text). Lane 1, total PLs from HEK293S cells; lanes 2–5, residual PL after purification and extensive washings in the detergents shown. Mobilities of control samples of phosphatidylserine (PS), phosphatidylcholine (PC), and phosphatidylethanolamine (PE) are shown. Quantitation of radioactive PC remaining is shown in Table 2.
