Table 1.
Stability of ROS rhodopsin after purification in different detergents and quantitation of phospholipids remaining with rhodopsin
| Detergent (concentration, %) | Stability, t1/2 | mol Pi per mol rhodopsin |
|---|---|---|
| DM (0.1) | >6 months | 1.4 |
| Cholate (2) | 600 h | 7.6 |
| OG (2) | 187 h | 4.4 |
| CHAPS (2) | 140 h | 3.6 |
| DM (0.1) | 110 h | 1.4 |
| Cholate (2) |
For details, see text. The amount of PL remaining with rhodopsin was determined by Pi analysis (see Materials and Methods). The amount of rhodopsin was determined spectrophotometrically.