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Characterization of residual 32P-labeled PL remaining with rhodopsin after purification in various detergents
| Detergent (concentration, %) | Rhodopsin, nmol | PL
|
mol PL per mol rhodopsin | |
|---|---|---|---|---|
| 32P recovered, cpm | nmol | |||
| DM (0.1) | 2.34 | 147 | 0.016 | 0.007 |
| Cholate (2) | 2.46 | 1,365 | 0.151 | 0.06 |
| OG (2) | 2.52 | 58 | 0.006 | 0.003 |
| CHAPS (2) | 1.92 | 1,196 | 0.132 | 0.07 |
The molar ratio of PL to rhodopsin was determined after calculating specific radioactivity of PC (9,030 cpm/nmol PC) as described in Materials and Methods.
