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. 2006 Oct 24;4(11):e363. doi: 10.1371/journal.pbio.0040363

Figure 4. Characterization of FWA siRNAs in Different Genetic Backgrounds.

Figure 4

(A) Schematic diagram of the FWA locus and position of LNA probe used to detect siRNAs.

(B) FWA siRNAs are still produced when the gene is unmethylated. siRNAs from wild type, fwa-1, and met1–3 were analyzed by Northern blotting. miR159 was probed as a loading control.

(C) FWA siRNAs from plants with the indicated genotype were analyzed by Northern blotting.

(D) siRNAs in fwa-1 do not cause mRNA destruction. The ratio of FWA to ACTIN7 mRNA levels in rosette leaf tissue was measured by RT-qPCR. Reverse transcription was performed with a poly-T oligonucleotide. There was no PCR amplification when reverse transcriptase was omitted (unpublished data).