Table 3.
Primary Antibodies, Dilutions, Incubation Conditions, and Antigen Retrieval Methods
| Antibody | Antigen | Source | Antigen retrieval | Dilution factor | Incubation conditions |
|---|---|---|---|---|---|
| 3F4 | PrP; aa 108 to 111 | DAKO | HA/FA | 1:50 | 4°C o/n or RT 60 min |
| R9 | PrP; aa 89 to 103 | R. Kascsak | FA | 1:400 | RT 60 min |
| R27 | PrP; aa 89 to 103 | R. Kascsak | FA | 1:400 | RT 60 min |
| MAB348 | Pre-A4 βAPP, aa 66 to 81 | Chemicon | FA | 1:250 | RT 90 mins |
aa, amino acid range of antigen; HA, hydrolytic autoclaving (121°C for 10 minutes in distilled water); FA, 96% formic acid; o/n, overnight; RT, room temperature.