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. 2004 Jul;165(1):147–157. doi: 10.1016/s0002-9440(10)63283-x

Figure 5.

Figure 5

Immunohistology demonstrates attenuated PMN infiltration into cardiac allografts in IFN-γ-blocked or -deleted recipients at 24 hours post-reperfusion. PMN infiltration into cardiac allografts was significantly reduced in wild-type recipients receiving IFN-γ-blocking mAb (XMG1.2) (c) pre- and post-transplant. PMN infiltration was also reduced in IFN-γ−/− recipients (d). Isograft (a) and allograft (b) sections taken at 24 hours post-reperfusion are shown for comparison. e: Quantification of PMN infiltration into cardiac isografts and allografts following at 24 hours post-reperfusion when IFN-γ is blocked or absent. Blocking INF-γ with a mAb (XMG1.2) resulted in attenuated PMN infiltration into cardiac allografts comparable to wild-type isografts (*, P < 0.01). Recipients with a gene deletion of IFN-γ showed similar results. PMN infiltration in recipients treated with anti-IFN-γ (XMG1.2) or in IFN-γ−/− recipients of cardiac isografts was unchanged from control-treated isograft recipients. All results are displayed as the mean and SD of at least 10 random myocardial fields from two non-consecutive sections from three to four grafts per group counted twice in a blinded fashion. The counts are normalized to tissue area and shown in mm2.