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. 2004 Jul;165(1):11–24. doi: 10.1016/S0002-9440(10)63271-3

Figure 1.

Figure 1

Endothelial life span is increased by hTERT. A: Passage 27 hTERT-HDLEC were photographed under phase contrast microscopy. The cells retain their typical cobblestone morphology and are contact inhibited. Bar, 100 μm. B: RT-PCR assay. Samples were run in a 1.5% agarose gel. The expected 350-bp band is indicated. Marker (1-kb ladder, Invitrogen), primary HDMVEC (negative control, lane 1), H20 (lane 2), RPE-hTERT (positive control, lane 3), hTERT-HDLEC, passage 9 (lane 4). C: Cell extracts were prepared and the TRAP assay was performed using the Intergen TRAPeze kit. Samples were run in an 8% acrylamide gel. Marker kit control, Mdx12 hTERT fibroblasts (positive control, lane 1), primary HDMVEC, passage 6 (lane 2), hTERT-HDLEC passage 9 (lane 3), H2O (lane 4).