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. 2004 Jul;165(1):259–271. doi: 10.1016/S0002-9440(10)63294-4

Figure 4.

Figure 4

The effect of stress on the percentage of hair follicle with TUNEL+ cells (mean ± SEM). A fluorescence microscope (Zeiss, Germany) with excitation emission filter systems for fluorescence induced by DAPI and FITC was used for the detection of apoptotic cells. A hair follicle was considered to be TUNEL+, if one or more apoptotic cell bodies were present. We then calculated the percentage of such TUNEL+ hair follicles to the total number of hair follicles. Mean percentages per group ± SEM are depicted in this Figure. Exposure to stress 2 days prior assessment led to a significant increase of apoptotic cells/hair follicle. The effect of stress on hair follicle apoptosis could be significantly abrogated by treatment with anti-NGF antibody. Significance of differences was determined by the Mann-Whitney U/Wilcoxon Rank test, ***, P < 0.001.