Figure 6.
ERK (A), JNK (B), and Akt (C) activation induced by BCR ligation in TSK/+ B cells. Splenic B cells purified from TSK/+ or wild-type control littermates (1 × 106/lane) were stimulated with anti-IgM. Subsequently, lysates of B cells were subjected to SDS-PAGE and transferred to nitrocellulose membranes for subsequent immunoblotting with phospho-specific Abs for the activated forms of the MAPKs and Akt. Results (left) are representative of those obtained with three littermate pairs of mice. Membranes were reprobed with anti-ERK2, anti-JNK1, and anti-Akt Abs. Values in the right graphs represent the relative mean optical density (±SEM) of band intensities determined by scanning densitometry from three independent immunoblotting experiments. Phosphorylation levels are shown as percentage of wild-type B cells at 0 minutes and 5 minutes were defined as 0% and 100%, respectively. *, <0.05; **, <0.01.