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. 2004 Aug;165(2):511–521. doi: 10.1016/S0002-9440(10)63316-0

Figure 1.

Figure 1

A: Pedigree of the family displaying both MTC and PTC and carrying the RET C634S mutation. Gray boxes, MTC; black boxes, PTC; arrow, index case. Patients carrying mutant or wild-type RET are indicated. B: Medullary (first line images) and papillary (second line images) thyroid carcinomas in the index patient (patient 2) stained with conventional H&E and immunostained with RET (RET), calcitonin (CALC), and thyroglobulin (TG) antibodies. The MTC in the first line images shows positive (brown) immunoreactivity for RET and calcitonin but no reactivity for thyroglobulin. The arrows point to nonneoplastic thyroid follicular epithelium that is positive for thyroglobulin but negative for RET and calcitonin, as expected. The PTC in the second line images shows positive (brown) immunoreactivity for RET and thyroglobulin but no reactivity for calcitonin. Omission of the primary antibody and competition with a molar excess of recombinant RET protein were used as negative controls (not shown).