Table 1.
Primers Selected for RET Analysis
| Name | Gene | Position* | Sequence | Primer size |
|---|---|---|---|---|
| TM(+) | RET | 1922–1941 | CTG TCC TCT TCT CCT TCA TC | 20 mer |
| TK1(+) | RET | 2149–2168 | TGG GAA TTC CCT CGG AAG AA | 20 mer |
| TK2(−) | RET | 2364–2383 | TGC AGG CCC CAT ACA ATT TG | 20 mer |
| TK3(−) | RET | 2185–2202 | TTC GCC TTC TCC TAG AGT | 18 mer |
| H4(+) | H4 | 335–351 | GCA AAG CCA GCG TTA CC | 17 mer |
| RFG(+) | RFG | 746–763 | CCC CAG GAC TGG CTT ATC | 18 mer |
| PTC2(+) | RIα | 602–619 | TAT CGC AGG AGA GAC TGT | 18 mer |
| PTC5(+) | Golgin | 1469–1486 | TAC TAG AAT ACT GCA ATC | 18 mer |
| PTC6(+) | HTIF1 | 1089–1106 | GCT CTA CTG CAT CAG TTA | 18 mer |
| PTC7(+) | HTIFγ | 1096–1113 | CAT TTT GCA GCT ACT CAG | 18 mer |
| PTC8(+) | KTN1 | 2760–2777 | ACA GGG AAG TGG TTA CAG | 18 mer |
*
The positions of TM, TK1, TK2, and TK3 are according to the standard RET sequence with the numeration beginning from the start codon of RET, according to this numeration the breakpoint in both RET/PTC1 and RET/PTC3 is at the nucleotide 2136; the positions of H4(+) (accession number M31213), RFG(+) (accession number X77548) and PTC2, 5, 6, 7, and 8 primers are according to current sequences of the NCBI database.