Table 1.
SAGE Libraries of Normal (N) ECs or Tumor ECs (T) Were Derived from Fresh Surgical Samples of Temporal Lobe Cortex or Glioma, Respectively
| Sample | Description | Tags generated | EC selection |
|---|---|---|---|
| Brain N1 | Normal temporal lobectomy ECs | 43,000 | P1H12 |
| Brain N2 | Normal temporal lobectomy ECs | 49,000 | UEA-I |
| Brain T1 | Grade IV glioma ECs | 46,000 | P1H12 |
| Brain T2 | Grade III glioma ECs | 50,000 | P1H12 |
| Brain T3 | Grade IV glioma ECs | 58,000 | UEA-I |
ECs were separated using magnetic beads coated with either an endothelial specific monoclonal antibody, P1H12, or an endothelial specific lectin, UEA-I, as indicated. Sequencing of each library was performed to the indicated tag depth.