Figure 10.

Co-expression of the 14-3-3 ε isoform and vimentin in cultured human astrocytes and reactive astrocytes in chronic demyelinating lesions of MS. Cultured human astrocytes and MS brain tissues were processed for double immunolabeling with anti-vimentin antibody and ε isoform-specific antibody or anti-GFAP antibody followed by labeling with fluorescein isothiocyanate- and rhodamine-conjugated secondary antibodies. a to f represent cultured human astrocytes (AS-BW) (a–d); no. 744, chronic active demyelinating lesions in the subcortical white matter of the frontal lobe (e, f); vimentin (a, c, e); ε (b, f); and GFAP (d).