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. 2004 Sep;165(3):1019–1032. doi: 10.1016/S0002-9440(10)63363-9

Figure 2.

Figure 2

ACE distribution and activity. A: Littermate wild-type (gray bars) and ACE 8/8 mice (black bars) were sacrificed and ACE activity was measured in organ homogenates. ACE activity is expressed as units per microgram total solubilized protein. ACE 8/8 mice have about 100-fold increased ACE activity in cardiac tissues. Significant activity is also seen in lung, testis, and plasma. In contrast, no ACE activity was detected in kidney homogenates of ACE 8/8 mice. The data are the average of 6 to 10 individual mice. All data presented are the group means ± SEM. <DL indicates less than detection limit. B: Tissue homogenates from wild-type (WT) and ACE 8/8 mice were studied by Western blot analysis after separation by SDS-PAGE. Somatic ACE is a band of about 170 kd while testis ACE is a band at about 95 kd. ACE 8/8 mice have substantially more somatic ACE in the whole heart as compared to wild-type mice. In contrast, these mice have somewhat less ACE in the lung and plasma than wild-type mice. ACE 8/8 mice have no ACE in the kidney. They also lack somatic ACE expression in the testis but do express typical levels of testis ACE.